Raw-blue cells

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August undefined, 2024

WebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions. WebTherefore, RAW-Blue™ cells can be used as: TLR reporter cells: they express all TLRs, with the exception of TLR5, at different levels NOD reporter cells: …

RAW-Dual™ cells IFN & NF-κB reporter macrophages - InvivoGen

WebJun 29, 2024 · RAW-Blue macrophages were purchased from InvivoGen (San Diego, CA). All cells were propagated in RPMI 1640 supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 °C in a 5% CO 2 incubator [16, 17]. Cell viability assay. Cell death was quantified by the MTT reduction assay. Cell viability was quantified by the Alamar … WebAug 24, 2010 · RAW-Blue cells are derived from RAW-264.7 macrophages that stably express a secreted embroyonic alkaline phosphotase (SEAP) gene which can be induced by NF-κB or AP-1 transcription factors. Treatment of RAW-Blue cells with Rx inhibited the LPS-induced NF-κB activity in a time- and dose-dependent manner as indicated by the reduced … laboratory underbench storage

RAW-Blue ISG Cells - InvivoGen

WebThe in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor ... WebJan 16, 2024 · Then you can follow this protocol: 1- Plate 1–2 * 10^4 adherent cells or 1–2 * 10^4 to 1 * 10^5 suspension cells in 100μl per well of a 96-well plate. Incubate overnight. 2- Allow the TR to ... WebRAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. InvivoGen has developed RAW-Blue ™ cells and RAW-Lucia ™ cells, which express a secreted inducible ... promo royal palace kirrwiller

What are the validation parameters for RAW BLUE ... - ResearchGate

RAW-Blue Cells Data sheet InvivoGen

WebDec 20, 2024 · The measured mTLR9 activity value of all the synthesized 24-mer ODNs in the dataset. The ODNs were divided into two groups of high (shown in purple) and low (shown in green) activity using a cutoff score of 0.4, based on the optimal density (OD) results from the Raw-blue reporter cell assay WebRAW-Blue™ cells endongenously express various PRRs, including TLRs (except TLR5), RIG-I, MDA-5, NOD1/2 and Dectin-1 at different levels. The PRR stimulation using specific agonists leads to the activation of NF-kB and AP-1 and, subsequently to the expression of the reporter gene encoding SEAP. RAW-Blue™ cells are selectable with Zeocin®. promo scarlett whiteningWebIn the present study, we used murine macrophage RAW-Blue cells, which are chromosomally integrated with secreted embryonic alkaline phosphatase (SEAP) inducible by NF-κB. We confirmed the expression of TLR4 mRNA and protein in RAW-Blue cells by RT-PCR and Western blot, respectively. laboratory trosor

"WebApr 22, 2015 · S2 Fig. RAW-BLUE cells detect trace levels of endotoxin in heparin lots. RAW-BLUE cells were cultured with 10 different lots of heparin (#1–10) in the presence or absence of 100pg/mL endotoxin for 24h. TNF-α levels were measured by ELISA in cell supernatant at 24 h. Each point represents mean ± SD of triplicate cell culture. " - Raw-blue cells

Raw-blue cells

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WebNF-κB activity from LPS-activated RAW-Blue™ cells after incubation with 30 and 300 μg/ml of acacia (AG), 30 and 300 μg/ml of guar gum (GG) and 30 μg/ml of 5ASA for 24 h in serum-free medium ... WebRAW-Blue™ cells. Catalog No. raw-sp. NF-κB-SEAP Reporter Cell Line. Pack Size. 3-7 x 10e6 cells. Quantity.

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WebNational Center for Biotechnology Information WebRAW-Blue cells were treated with the shown concentrations of FP20 and incubated for 16–18 h. MPLA and LPS (100 ng/mL) were used as controls. The 100% stimulation has been assigned to the positive control LPS. Data are expressed as mean ± SEM of at least three independent experiments ...

WebRAW-Blue™ ISG cells allow the detection of bioactive murine type I IFNs or PAMPs by monitoring the activation of the interferon regulatory factor (IRF) pathway. They are derived from the RAW 264.7 macrophages. RAW-Blue™ ISG cells stably express a secreted embryonic alkaline phosphatase (SEAP) gene under the control of the interferon-stimulated WebOct 1, 2024 · Results to characterize the IFN response time of the RAW-Blue ISG ™ (RAW-Blue) and B16-BlueIFN-α/β™ (B16-Blue) cells to IFNα and IFNβ and SEAP catalyzed Quantiblue™ (QB) development time are shown in Fig. 1, Fig. 2, Fig. 3.In Fig. 1 a range of IFN concentrations of 1 to 10,000 Unit/ml (U/ml) were added to each cell type and incubated …

WebJun 28, 2024 · QUANTI-Blue colorimetric enzyme assay: RAW-Blue cells were added at 1 × 10 5 cells per well in a 96-well plate and sequentially the same amounts of different particles at a particle: cell ratio ... WebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ...

WebNational Center for Biotechnology Information

WebRAW-Blue™ cells were derived from the murine RAW 264.7 macrophages with chromosomal integration of a SEAP reporter construct inducible by NF-κB. RAW-Blue™ cells express many pattern-recognition receptors (PRRs), including all toll-like receptors (TLRs) except for TLR5, but with TLR4 and TLR8 expressed at the highest levels. promo sawer alfamartWebCo-immunoprecipitation experiments using cell lysates from RAW-blue cells further dem- onstrated that Neu1 forms a complex with TLR9 receptors in naive and ODN treatment of cells for 30 min (Fig. 4C). laboratory vacancies in port elizabethWebRAW‑Dual™ cells stably express two reporter genes encoding SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase . SEAP expression depends on the activation of the endogenous MIP-2 promoter. MIP‑2, also known as CXCL2, is the murine homolog of human IL-8, a chemokine produced in an NF-κB-dependent manner [3]. promo sg.apmarketingap.comWebJun 5, 2013 · RAW-Blue™ cells are permanently transfected with an NF-κB- and AP-1-sensitive promoter-driven alkaline phosphatase. NF-κB and AP-1 binds to the promoter upon nuclear translocation, and induces the expression of alkaline phosphatase that is detected by a dye-based assay and a plate reader. promo scotts of stowWebSize. 3-7 x 10e6 cells. Contact us. Ask RAW ™ cells selection audit. Properties. Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water. Conjugation. Blue Substrate. laboratory unit fire hazard classWebJun 30, 2011 · During the course of blocking CD36 oxLDL binding function using anti CD36 antibodies, we have identified a novel domain of CD36 that triggers inflammatory response-independent of oxLDL binding. OxLDL bound to the mouse reporter cell line RAW-Blue induced TNF-α and RANTES mRNA and protein expression. Pretreatment of RAW-Blue … promo screen marrickville promo scrabble word