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Lamp2 western blot

Webb14 maj 2024 · This suggests that LAMP2, which consists of a large intraluminal domain in addition to the transmembrane part and a small cytosolic domain, is protected from proteolysis inside a vesicle. A... WebbLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome.

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WebbLAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C. Database link: P13473 (Peptide available as ab23322) Positive control WB: Human liver tissue lysate. ICC: CaCo2 cells, HeLa, MCF7 and HepG2 cells. IHC-P: Human breast adenocarcinoma tissue. General notes The Life Science industry has been in the grips of a reproducibility crisis for a … WebbWe next characterized molecules in sub-RPE deposits in Lamp2 KO mice by Western blotting. Key proteins found in human drusen, such as apolipoprotein E (APOE), … eye power exercise https://janak-ca.com

Restoration of lysosomal function after damage is …

WebbObjective: Autophagy is a highly conserved degradation pathway in cells, which has been involved in many physiological processes and implicated in human age-related diseases. However, autophagy activities have not been systemically investigated with human tissues and cells. Methods: Lysosomal associated membrane protein-2 (LAMP-2) protein is … WebbFigure 1 The expression of PHLPP in bortezomib-resistant MM cells. (A) Western blot analyses of the expression of PHLPP, LAMP2, and key autophagy signaling molecules … does a sauna help sweat out toxins

Up-Regulated Expression of LAMP2 and Autophagy Activity …

Category:Lumenal Galectin-9-Lamp2 interaction regulates lysosome and

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Lamp2 western blot

Anti-LAMP2b Western Blot Antibody Products Biocompare

WebbAnti-LAMP2B antibody - Lysosome Marker Applications: Western Blot (WB) Reactivity: Human (Hu) Conjugate/Tag: Unconjugated Quantity: 100 UG Citations: (26) Supplier … WebbExosome quality was assessed by Coomassie staining and Western blotting for CD9, LAMP2 exosomal markers and a negative marker Grp94. Results: Correlation between …

Lamp2 western blot

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Webb7 feb. 2024 · Lamp2 overexpression promotes resistance against OGD stress in cardiomyocytes. (A) Western blotting was performed to detect Lamp1 and Lamp2 levels after OGD treatment for different periods. (B) … WebbSummary. LAMP2 antibody detects lysosome-associated membrane protein 2 (LAMP-2, or CD107b), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP-2 has a similar distribution to LAMP-1 in endolysosomal and autophagic organelles. Moreover, like LAMP-1, LAMP-2 has been used to study …

Webb27 aug. 2024 · e Co-immunoprecipitation of Gal-9 and Lamp2 or M6PR in CMT93 cells, analyzed by Western blot analysis. f Lysosomal hydrolase activity of the indicated … WebbWestern Blotting Protocol. For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. NOTE: Please refer to primary antibody …

WebbAdditional Information: LAMP2 is a marker of lysosmes and late endosomes. ABL-93 is directed against the luminal domain of LAMP-2 common to all isoforms. RRID: … WebbDetection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, JEG-3 human epithelial choriocarcinoma cell …

WebbLAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C. Database link: P13473 (Peptide available as ab23322) Positive control WB: Human liver tissue lysate. ICC: CaCo2 cells, HeLa, MCF7 and HepG2 cells. IHC-P: …

WebbWe next characterized molecules in sub-RPE deposits in Lamp2 KO mice by Western blotting. Key proteins found in human drusen, such as apolipoprotein E (APOE), clusterin (CLU), and vitronectin (VTN ... eye power improve tipsWebbGalectin Therapeutics hpde cells Hpde Cells, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. does a savings account build creditWebb10 dec. 2015 · Western blot of membrane protein extracted from AA and NA MCF-7 cells shows higher expression of LAMP2 at the cell surface of AA cells. ( d ) Schematic presentation of LAMP2 role in acid ... eye popping makeup for brown eyesWebbThe blot was incubated with ab18529 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406 . LAMP2B undergoes extensive glycosylation so ab18529 detects a band of 110 kDa by Western blot. does a sauna lower blood pressureWebbIn age-related macular degeneration (AMD), hydroquinone (HQ)-induced oxidative damage in retinal pigment epithelium (RPE) is believed to be an early event contributing to dysregulation of inflammatory cytokines and vascular endothelial growth factor (VEGF) homeostasis. However, the roles of antioxidant mechanisms, such as autophagy and … does a sauna raise or lower blood pressureWebbFigure 1 The expression of PHLPP in bortezomib-resistant MM cells. (A) Western blot analyses of the expression of PHLPP, LAMP2, and key autophagy signaling molecules in bone marrow samples from patients with MM (upper), and quantification of the bands (lower).(B) Western blot analyses of the expression of PHLPP, LAMP2, and key … does a savings account have a routing numberWebbLysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). does a savings bond increase in value