Web• Highly immunogenic—KLH has a high molecular mass (4.5 x 10 5 to 1.3 x 10 7 Daltons; aggregates of 350 and 390 kDa subunits) and elicits a stronger immune response than …
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WebKLH contains an abundance of functional groups available for conjugation with hapten molecules. On a per-mole basis (using an average multi-subunit MW of 5,000,000 … WebProperties: Chemical Name: Phosphorylcholine Keyhole Limpet Hemocyanin Formula: C11H17N3O4P-KLH Molecular Weight: 286.25 Appearance: orange powder Absorption Maximum (Lambda Max): 475 Extinction Coefficient at Lambda max: 12600 Product usage:
WebMar 29, 2024 · Buy TNP-KLH (Keyhole Limpet Hemocyanin), TNP hapten conjugated to KLH protein via amide bonds to lysine, from Santa Cruz. MF: C6H3N4O6-KLH. ... determined at 348 nm using the extinction coefficient of TNP and is given as a molar ratio of TNP to KLH assuming the molecular weight of KLH is 100,000. Appearance : Powder. Physical State : ... WebKeyhole limpet hemocyanin (KLH), a carrier protein with a molecular weight ranging from 3,000,000-7,500,000, has many available primary amines to facilitate protein conjugation. …
WebKLH Antibody (600-405-466) in WB Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Web[ 1] Hemocyanin is a high molecular weight copper containing glycoprotein. It reversibly binds oxygen and forms the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, also called the giant keyhole limpet. It is native to the southern California coast and Mexico.
WebBreast cancer is the most common invasive cancer diagnosed among women. A cancer vaccine has been recognized as a form of immunotherapy with a prominent position in the prevention and treatment of breast cancer. The majority of current breast cancer vaccination strategies aim to stimulate antitumor T-cell responses of the HER2/neu oncogene, which …
WebMar 8, 2013 · Total Structure Weight: 784.8 kDa Atom Count: 55,292 Modelled Residue Count: 6,796 Deposited Residue Count: 6,796 Unique protein chains: 2 Display Files … css style buttonWebOct 25, 2024 · HMW-KLH is the native KLH protein consisting of multiple subunits with a size of approximately 4–8 MDa. Each subunit has a size of approximately 350–390 kDa. HMW … css style checkbox imageThere are two keyhole limpet hemocyanin genes, termed KLH1 and KLH2 which share around 60% identity at the protein level. Both encode large glycosylated proteins consisting of around 3400 amino acids and a molecular weight of around 390,000 Daltons, excluding the glycosylation. The protein oligomerises to … See more Keyhole limpet hemocyanin (KLH) is a large, multisubunit, oxygen-carrying, metalloprotein that is found in the hemolymph of the giant keyhole limpet, Megathura crenulata, a species of keyhole limpet that lives off … See more KLH is purified from the hemolymph of Megathura crenulata by a series of steps that typically includes ammonium sulfate precipitation and dialysis, and may involve chromatographic purification to obtain the highest purity. KLH purification may also include See more Haptens can be coupled to KLH using several methods. A simple one-step coupling can be performed using the carbodiimide crosslinker EDC to covalently attach carboxyls to … See more KLH is being tested in a variety of cancer vaccines, including non-Hodgkin's lymphoma, cutaneous melanoma, breast and bladder cancer. These vaccines contain specific See more Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. Haptens are … See more • Concholepas concholepas hemocyanin (marketed as Blue Carrier) • Bovine serum albumin (BSA) • Cationized BSA (cBSA) See more Because of the beneficial uses of KLH, the Giant Keyhole Limpet, from which KLH is derived, has been a subject of growing concern among … See more css style checkbox labelWeboxygen transport metalloproteins. Keyhole Limpet Hemocyanin (KLH), from the hemolymph of the marine mollusc Megathura crenulata, is expressed as two subunit isoforms (KLH1 … css style clear bothWebcounted the molar conjugation ratio of NP/KLH taking the molecular weight of KLH as 5,000,000. Preparation of antigen primed lymphocytes. Antigen primed lymphocytes were col lectedusing a modification of Alkan's method (1). In brief, 100ƒÊg of NP-KLH, GLƒÓ, or KLH in Freund's complete adjuvant (Difco Laboratories, Detroit, Michi css style class selectorWebKeyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with … early 1900s shipsWebPotassium Hydride molecular weight. Molar mass of KH = 40.10624 g/mol. Convert grams Potassium Hydride to moles or moles Potassium Hydride to grams. Molecular weight … css style background image